Other factors which affect rate of enzyme action are 1.
If there is no hydrogen peroxide present, the catalase cannot function, which is why in cup one you shouldn't have seen any bubble or foam production. However, the dish soap that you added to all your solutions is able to trap the gas bubbles, which results in the formation of a stable foam.
Dilute immediately before use and put in a clean brown bottle, because dilution also dilutes the decomposition inhibitor. Catalase is a very common enzyme that is present in almost all organisms that are exposed to oxygen.
In this activity you will disarm hydrogen peroxide with the help of catalase from yeast. Put the syringe in place in the bung of the flask, but do not push the plunger straight away.
Role in Living Organisms Although its ability to break down a toxic molecule such as hydrogen peroxide might make catalase seem to be an indispensable commodity, mice engineered to develop without catalase have a normal physical appearance.
From your graph of activity against temperature, determine the optimum temperature. Each enzyme has an active site, which is where the reaction takes place.
Invert it over the trough of water, with the open end under the surface of the water in the bowl, and with the end of the rubber tubing in the measuring cylinder.